Add HUMAnN 4 database generation protocols - #1
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Pull request overview
Adds a set of draft, AI-agent-compatible protocols documenting the end-to-end workflow for generating HUMAnN 4 databases (SGB aggregation → augmented clustering → ChocoPhlAn build → translated search build → utility mapping).
Changes:
- Adds a protocol for aggregating and subsampling MetaPhlAn 4.2 SGB genomes/MAGs.
- Adds a protocol for augmented UniRef-compatible ORF clustering and ORF→cluster mapping generation.
- Adds protocols for building ChocoPhlAn nucleotide pangenomes, a DIAMOND translated-search database, and functional utility mapping TSVs.
Reviewed changes
Copilot reviewed 5 out of 5 changed files in this pull request and generated 5 comments.
Show a summary per file
| File | Description |
|---|---|
| protocols/humann4-sgb-aggregation/protocol.md | Defines the SGB genome retrieval and subsampling step that seeds the HUMAnN 4 DB build pipeline. |
| protocols/humann4-augmented-clustering/protocol.md | Documents ORF prediction, UniRef alignment, de novo clustering, and ORF mapping generation for novel clusters. |
| protocols/humann4-chocophlan-build/protocol.md | Describes constructing per-SGB nucleotide pangenomes and Bowtie2 indexes for ChocoPhlAn. |
| protocols/humann4-translated-search-build/protocol.md | Documents building the merged UniRef90 + novel-cluster protein database and DIAMOND index. |
| protocols/humann4-utility-mapping/protocol.md | Describes generating HUMAnN mapping TSVs by combining UniProt cross-references and eggNOG-mapper outputs. |
Suppressed comments (4)
protocols/humann4-utility-mapping/protocol.md:32
- Remove trailing whitespace from the Markdown heading to avoid markdownlint/formatting noise.
## Materials
protocols/humann4-translated-search-build/protocol.md:32
- Remove trailing whitespace from the Markdown heading to avoid markdownlint/formatting noise.
## Materials
protocols/humann4-chocophlan-build/protocol.md:32
- Remove trailing whitespace from the Markdown heading to avoid markdownlint/formatting noise.
## Materials
protocols/humann4-augmented-clustering/protocol.md:32
- Remove trailing whitespace from the Markdown heading to avoid markdownlint/formatting noise.
## Materials
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Drafts the 5 ai-agent-compatible protocols required to generate HUMAnN 4 databases.