Symbiont Identity Impacts Prokaryotic Microbiome Dynamics During Heat Stress in a Model System for Corals

About

This GitHub repository contains the code and part of the data to recreate the analyses of:

Glynn, V.M., Lawrence, E.C., Cleves, P.A., Barrett, R.D.H. (2026) Symbiont Identity Impacts Prokaryotic Microbiome Dynamics During Heat Stress in a Model System for Corals. Integrative and Comparative Biology in press.

Contents

This repository contains two folders:

• "16S" : R scripts to generate amplicon sequence variants (ASVs) from our 16S marker-gene data using DADA2 (Callahan et al., 2016), the resulting RDS file from DADA2, and beta diversity analyses of this data, alongside analyses of algal cell density declines as a function of temperature.

• "Algal density" : Aiptasia algal flow cytometry and protein concentration data over the course of our heat stress assay.

Questions

If you have questions about the scripts please contact Dr. Victoria Marie Glynn (victoria.glynn@mail.mcgill.ca).