diff --git a/app/controllers/sample_manifest_upload_with_tag_sequences_controller.rb b/app/controllers/sample_manifest_upload_with_tag_sequences_controller.rb
index b863ccc488..6b1d0be464 100644
--- a/app/controllers/sample_manifest_upload_with_tag_sequences_controller.rb
+++ b/app/controllers/sample_manifest_upload_with_tag_sequences_controller.rb
@@ -19,7 +19,10 @@ def create
   end
 
   def create_uploader
-    SampleManifest::Uploader.new(params[:upload], SampleManifestExcel.configuration, current_user, params[:override])
+    samples = params[:override_samples].present?
+
+    overrides = { samples:, exclude_fields: }
+    SampleManifest::Uploader.new(params[:upload], SampleManifestExcel.configuration, current_user, overrides)
   end
 
   def upload_manifest
@@ -82,4 +85,11 @@ def prepare_manifest_pagination # rubocop:todo Metrics/MethodLength
     @display_manifests = pending_sample_manifests | completed_sample_manifests
     @sample_manifests = SampleManifest.paginate(page: params[:page])
   end
+
+  def exclude_fields
+    excluded_fields = []
+    excluded_fields << :volume if params[:overwrite_volume].blank?
+    excluded_fields << :concentration if params[:overwrite_concentration].blank?
+    excluded_fields.compact
+  end
 end
diff --git a/app/models/sample_manifest.rb b/app/models/sample_manifest.rb
index 7b9525715a..17b29c231a 100644
--- a/app/models/sample_manifest.rb
+++ b/app/models/sample_manifest.rb
@@ -51,7 +51,7 @@ def self.included(base)
   has_uploaded_document :uploaded, differentiator: 'uploaded'
   has_uploaded_document :generated, differentiator: 'generated'
 
-  attr_accessor :override, :only_first_label, :barcode_type
+  attr_accessor :overrides, :only_first_label, :barcode_type
   attr_writer :rows_per_well, :invalid_wells
 
   class_attribute :spreadsheet_offset
diff --git a/app/models/sample_manifest/uploader.rb b/app/models/sample_manifest/uploader.rb
index 4a165aa5a9..6198e61de7 100644
--- a/app/models/sample_manifest/uploader.rb
+++ b/app/models/sample_manifest/uploader.rb
@@ -9,7 +9,7 @@
 class SampleManifest::Uploader
   include ActiveModel::Validations
 
-  attr_reader :file, :configuration, :tag_group, :upload, :user, :override
+  attr_reader :file, :configuration, :tag_group, :upload, :user
 
   validates :tag_group, presence: { message: 'is not correctly configured for manifest generation' }
   validates :file, :configuration, :user, presence: true
@@ -18,14 +18,12 @@ class SampleManifest::Uploader
 
   delegate :processed?, :study, to: :upload
 
-  def initialize(file, configuration, user, override)
+  def initialize(file, configuration, user, overrides)
     @file = file
     @configuration = configuration || SequencescapeExcel::NullObjects::NullConfiguration.new
     @user = user
-    @override = override
     @tag_group = create_tag_group
-    @upload =
-      SampleManifestExcel::Upload::Base.new(file: file, column_list: self.configuration.columns.all, override: override)
+    @upload =  SampleManifestExcel::Upload::Base.new(file:, column_list:, overrides:)
   end
 
   def run! # rubocop:disable Metrics/MethodLength
@@ -54,6 +52,10 @@ def run! # rubocop:disable Metrics/MethodLength
 
   private
 
+  def column_list
+    configuration.columns.all
+  end
+
   def process_upload_and_callbacks
     return false unless upload.process(tag_group)
 
diff --git a/app/sample_manifest_excel/sample_manifest_excel/upload/base.rb b/app/sample_manifest_excel/sample_manifest_excel/upload/base.rb
index 3035f244a6..9d58f41c79 100644
--- a/app/sample_manifest_excel/sample_manifest_excel/upload/base.rb
+++ b/app/sample_manifest_excel/sample_manifest_excel/upload/base.rb
@@ -19,7 +19,7 @@ class Base # rubocop:todo Metrics/ClassLength
       include AccessionHelper
       include ActiveModel::Model
 
-      attr_accessor :file, :column_list, :start_row, :override
+      attr_accessor :file, :column_list, :start_row, :overrides
 
       # rubocop:todo Layout/LineLength
       attr_reader :spreadsheet, :columns, :sanger_sample_id_column, :rows, :sample_manifest, :data, :processor, :cache # TODO: probably shouldn't add the cache here, do it another way
@@ -47,7 +47,7 @@ def initialize(attributes = {}) # rubocop:todo Metrics/AbcSize
         @cache = Cache.new(self)
         @rows = Upload::Rows.new(data, columns, @cache)
         @sample_manifest = derive_sample_manifest
-        @override = override || false
+        @overrides = overrides || { samples: false, exclude_fields: [] }
         @processor = create_processor
       end
 
diff --git a/app/sample_manifest_excel/sample_manifest_excel/upload/processor/base.rb b/app/sample_manifest_excel/sample_manifest_excel/upload/processor/base.rb
index e1e4156fa4..4119e7d51d 100644
--- a/app/sample_manifest_excel/sample_manifest_excel/upload/processor/base.rb
+++ b/app/sample_manifest_excel/sample_manifest_excel/upload/processor/base.rb
@@ -49,7 +49,7 @@ def samples_valid?
           all_valid = true
 
           upload.rows.each do |row|
-            unless row.validate_sample
+            unless row.validate_sample(upload.overrides)
               upload.errors.add(:base, row.errors.full_messages.join(', ').to_s)
               all_valid = false
             end
@@ -60,7 +60,7 @@ def samples_valid?
 
         def update_samples_and_aliquots(tag_group)
           upload.rows.each do |row|
-            row.update_sample(tag_group, upload.override)
+            row.update_sample(tag_group, upload.overrides)
             substitutions.concat(row.aliquots.filter_map(&:substitution_hash)) if row.reuploaded?
           end
           update_downstream_aliquots unless no_substitutions?
diff --git a/app/sample_manifest_excel/sample_manifest_excel/upload/processor/multiplexed_library_tube.rb b/app/sample_manifest_excel/sample_manifest_excel/upload/processor/multiplexed_library_tube.rb
index faff473a9c..8eb3025cd2 100644
--- a/app/sample_manifest_excel/sample_manifest_excel/upload/processor/multiplexed_library_tube.rb
+++ b/app/sample_manifest_excel/sample_manifest_excel/upload/processor/multiplexed_library_tube.rb
@@ -24,7 +24,7 @@ def run(tag_group)
 
         def update_samples_and_aliquots(tag_group)
           upload.rows.each do |row|
-            row.update_sample(tag_group, upload.override)
+            row.update_sample(tag_group, upload.overrides)
             row.transfer_aliquot # Requests are smart enough to only transfer once
             substitutions.concat(row.aliquots.filter_map(&:substitution_hash)) if row.reuploaded?
           end
diff --git a/app/sample_manifest_excel/sample_manifest_excel/upload/row.rb b/app/sample_manifest_excel/sample_manifest_excel/upload/row.rb
index cd83d4218a..3cc1783e9c 100644
--- a/app/sample_manifest_excel/sample_manifest_excel/upload/row.rb
+++ b/app/sample_manifest_excel/sample_manifest_excel/upload/row.rb
@@ -88,17 +88,18 @@ def specialised_fields
       # - Updating all of the specialised fields in the aliquot
       # - Updating the sample metadata
       # - Saving the asset, metadata and sample
-      def update_sample(tag_group, override)
+      def update_sample(tag_group, overrides)
         return unless valid?
 
         @reuploaded = sample.updated_by_manifest
 
-        if skip_sample_update?(override)
+        if skip_sample_update?(overrides)
           @sample_skipped = true
           return
         end
 
-        @sample_updated = save_sample(tag_group)
+        exclude_fields = overrides[:exclude_fields]
+        @sample_updated = save_sample(tag_group, exclude_fields)
       end
 
       def changed?
@@ -106,12 +107,22 @@ def changed?
           aliquots.any? { |a| a.previous_changes.present? }
       end
 
-      def update_specialised_fields(tag_group)
-        specialised_fields.each { |specialised_field| specialised_field.update(tag_group:) }
+      def update_specialised_fields(tag_group, exclude_fields)
+        specialised_fields.each do |specialised_field|
+          puts "Exclude specialised fields: #{exclude_fields.inspect}, Processing column: #{specialised_field.name.to_sym}"
+          next if exclude_fields.include?(specialised_field.name.to_sym)
+
+          puts "Updating specialised field: #{specialised_field.name}"
+
+          specialised_field.update(tag_group:)
+        end
       end
 
-      def update_metadata_fields
+      def update_metadata_fields(exclude_fields)
         columns.with_metadata_fields.each do |column|
+          puts "Exclude metadata fields: #{exclude_fields.inspect}, Processing column: #{column.name}"
+          next if exclude_fields.include?(column.name.to_sym)
+
           value = at(column.number)
           column.update_metadata(metadata, value) if value.present?
         end
@@ -172,9 +183,9 @@ def labware
         sample.primary_receptacle.labware
       end
 
-      def validate_sample
+      def validate_sample(overrides)
         check_sample_present
-        sample_can_be_updated
+        sample_can_be_updated(overrides)
         errors.empty?
       end
 
@@ -247,12 +258,14 @@ def sanger_sample_id_exists?
       #
       # This confirms that the sample can be updated by attempting to apply the updates
       # and raising errors if any of the updates are invalid. It does not (seem to!) save any changes to the database.
-      def sample_can_be_updated
+      def sample_can_be_updated(overrides)
         return unless errors.empty?
 
+        exclude_fields = overrides[:exclude_fields]
+
         check_primary_receptacle
-        check_specialised_fields
-        check_sample_metadata
+        check_specialised_fields(exclude_fields)
+        check_sample_metadata(exclude_fields)
       end
 
       def check_primary_receptacle
@@ -261,19 +274,21 @@ def check_primary_receptacle
         errors.add(:base, "#{row_title} Does not have a primary receptacle.")
       end
 
-      def check_specialised_fields
+      def check_specialised_fields(exclude_fields)
         return unless errors.empty?
 
         specialised_fields.each do |specialised_field|
+          next if exclude_fields.include?(specialised_field.name.to_sym)
+
           unless specialised_field.valid?
             errors.add(:base, "#{row_title} #{specialised_field.errors.full_messages.join(', ')}")
           end
         end
       end
 
-      def check_sample_metadata
+      def check_sample_metadata(exclude_fields)
         # it has to be called here, otherwise metadata errors will not appear
-        update_metadata_fields
+        update_metadata_fields(exclude_fields)
         return if metadata.valid?
 
         errors.add(:base, "#{row_title} #{metadata.errors.full_messages.join(', ')}")
@@ -302,10 +317,10 @@ def link_tag_groups_and_indexes(fields)
         fields.each { |field| field.link(indexed_fields) }
       end
 
-      def save_sample(tag_group)
-        update_specialised_fields(tag_group)
+      def save_sample(tag_group, exclude_fields)
+        update_specialised_fields(tag_group, exclude_fields)
         asset.save!
-        update_metadata_fields
+        update_metadata_fields(exclude_fields)
         metadata.save!
         sample.updated_by_manifest = true
         sample.empty_supplier_sample_name = false
diff --git a/app/views/sdb/sample_manifests/_upload.html.erb b/app/views/sdb/sample_manifests/_upload.html.erb
index c3bfdcfba6..448c16d31f 100644
--- a/app/views/sdb/sample_manifests/_upload.html.erb
+++ b/app/views/sdb/sample_manifests/_upload.html.erb
@@ -6,7 +6,21 @@
         <%= file_field_tag :upload, required: true %>
       </div>
       <div class="form-group">
-        <%= check_box_tag :override %>&nbsp;<%= label_tag :override, 'Override previously uploaded samples' %>
+        <div class="form-check">
+          <%= check_box_tag :override_samples, class: 'form-check-input'%>
+          <%= label_tag :override_samples, 'Override previously uploaded samples', class: 'form-check-label' %>
+        </div>
+
+        <div class="ml-3"><%# Indent the volume and concentration checkboxes, since they depend on override_samples %>
+          <div class="form-check">
+            <%= check_box_tag :overwrite_volume, class: 'form-check-input', disabled: true %>
+            <%= label_tag :overwrite_volume, 'Overwrite volume', class: 'form-check-label' %>
+          </div>
+          <div class="form-check">
+            <%= check_box_tag :overwrite_concentration, class: 'form-check-input', disabled: true %>
+            <%= label_tag :overwrite_concentration, 'Overwrite concentration', class: 'form-check-label' %>
+          </div>
+        </div>
       </div>
       <div class="form-group mb-0">
         <%= submit_tag 'Upload manifest', class: 'btn btn-success' %>
@@ -14,3 +28,21 @@
     </fieldset>
   <% end -%>
 <% end %>
+
+<script>
+  // Add JavaScript to enable/disable the volume and concentration checkboxes based on the state of the override-samples checkbox
+  document.addEventListener('DOMContentLoaded', function() {
+    const overrideSamples = document.getElementById('override_samples');
+    const overwriteVolume = document.getElementById('overwrite_volume');
+    const overwriteConcentration = document.getElementById('overwrite_concentration');
+
+    // Enable the vol and conc checkboxes when override-samples is checked, otherwise disable them
+    function updateCheckboxStates() {
+      overwriteVolume.disabled = !overrideSamples.checked;
+      overwriteConcentration.disabled = !overrideSamples.checked;
+    }
+
+    // Listen for changes
+    overrideSamples.addEventListener('change', updateCheckboxStates);
+  });
+</script>
diff --git a/app/views/shared/metadata/show/_sample.html.erb b/app/views/shared/metadata/show/_sample.html.erb
index 17cb2a0196..8b7721a9b3 100644
--- a/app/views/shared/metadata/show/_sample.html.erb
+++ b/app/views/shared/metadata/show/_sample.html.erb
@@ -9,12 +9,15 @@
     <%= metadata_fields.plain_value(:geographical_region) %>
     <%= metadata_fields.plain_value(:ethnicity) %>
     <%= metadata_fields.plain_value(:dna_source) %>
-    <%= metadata_fields.plain_value(:volume) %>
     <%= metadata_fields.plain_value(:mother) %>
     <%= metadata_fields.plain_value(:father) %>
     <%= metadata_fields.plain_value(:replicate) %>
     <%= metadata_fields.plain_value(:collected_by) %>
 
+    <!-- Physical properties -->
+    <%= metadata_fields.plain_value(:volume) %>
+    <%= metadata_fields.plain_value(:concentration) %>
+
     <%# Fields for 'Next-gen sequencing' %>
     <%= metadata_fields.plain_value(:organism) %>
     <%= metadata_fields.plain_value(:gc_content) %>
diff --git a/spec/sample_manifest_excel/upload/processor_spec.rb b/spec/sample_manifest_excel/upload/processor_spec.rb
index 8f8a97b21e..e50f13d24b 100644
--- a/spec/sample_manifest_excel/upload/processor_spec.rb
+++ b/spec/sample_manifest_excel/upload/processor_spec.rb
@@ -38,6 +38,7 @@ def cell(row, column)
     let(:new_test_file_name) { 'new_test_file.xlsx' }
     let(:test_file) { Rack::Test::UploadedFile.new(Rails.root.join(test_file_name), '') }
     let(:tag_group) { create(:tag_group) }
+    let(:overrides) { { samples:, exclude_fields: } }
 
     before do
       allow(PlateBarcode).to receive(:create_barcode).and_return(build(:plate_barcode))
@@ -47,7 +48,7 @@ def cell(row, column)
     after { FileUtils.rm_f(test_file_name) }
 
     shared_examples 'it updates downstream aliquots' do |rows, columns|
-      it 'will update the aliquots downstream if aliquots data has changed and override is set to true' do
+      it 'will update the aliquots downstream if aliquots data has changed and override is set to overall all' do
         cell(rows.first, columns[:insert_size_from]).value = '100'
         cell(rows.last, columns[:insert_size_to]).value = '1000'
         download.save(new_test_file_name)
@@ -56,7 +57,7 @@ def cell(row, column)
             file: new_test_file,
             column_list: column_list,
             start_row: 9,
-            override: true
+            override: overrides
           )
         processor = described_class.new(reupload2)
         processor.update_samples_and_aliquots(tag_group)
@@ -65,7 +66,7 @@ def cell(row, column)
         expect(processor).to be_downstream_aliquots_updated
       end
 
-      it 'will update the aliquots downstream if tags were swapped and override is set to true' do
+      it 'will update the aliquots downstream if tags were swapped and override is set to overall all' do
         i7_tag1 = cell(rows.first, columns[:i7]).value
         i7_tag2 = cell(rows.last, columns[:i7]).value
         cell(rows.first, columns[:i7]).value = i7_tag2
@@ -76,7 +77,7 @@ def cell(row, column)
             file: new_test_file,
             column_list: column_list,
             start_row: 9,
-            override: true
+            override: overrides
           )
         processor = described_class.new(reupload2)
         processor.update_samples_and_aliquots(tag_group)
@@ -95,7 +96,7 @@ def cell(row, column)
             file: new_test_file,
             column_list: column_list,
             start_row: 9,
-            override: true
+            override: overrides
           )
         processor = described_class.new(reupload2)
         processor.update_samples_and_aliquots(tag_group)
@@ -110,7 +111,7 @@ def cell(row, column)
             file: new_test_file,
             column_list: column_list,
             start_row: 9,
-            override: true
+            override: overrides
           )
         processor = described_class.new(reupload2)
         processor.update_samples_and_aliquots(tag_group)
@@ -120,7 +121,7 @@ def cell(row, column)
     end
 
     shared_examples 'it updates chromium aliquots' do |rows, columns|
-      it 'will update the aliquots downstream if aliquots data has changed and override is set to true' do
+      it 'will update the aliquots downstream if aliquots data has changed and override is set to overall all' do
         cell(rows.first, columns[:insert_size_from]).value = '100'
         cell(rows.last, columns[:insert_size_to]).value = '1000'
 
@@ -130,7 +131,7 @@ def cell(row, column)
             file: new_test_file,
             column_list: column_list,
             start_row: 9,
-            override: true
+            override: overrides
           )
         processor = described_class.new(reupload2)
         processor.update_samples_and_aliquots(tag_group)
@@ -140,7 +141,7 @@ def cell(row, column)
         expect(processor).to be_downstream_aliquots_updated
       end
 
-      it 'will update the aliquots downstream if tags were swapped and override is set to true' do
+      it 'will update the aliquots downstream if tags were swapped and override is set to overall all' do
         chromium_tag1 = cell(rows.first, columns[:chromium_tag_well]).value
         chromium_tag2 = cell(rows.last, columns[:chromium_tag_well]).value
         cell(rows.first, columns[:chromium_tag_well]).value = chromium_tag2
@@ -151,7 +152,7 @@ def cell(row, column)
             file: new_test_file,
             column_list: column_list,
             start_row: 9,
-            override: true
+            override: overrides
           )
         processor = described_class.new(reupload2)
         processor.update_samples_and_aliquots(tag_group)
@@ -174,7 +175,7 @@ def cell(row, column)
             file: new_test_file,
             column_list: column_list,
             start_row: 9,
-            override: true
+            override: overrides
           )
         processor = described_class.new(reupload2)
 
@@ -231,7 +232,7 @@ def cell(row, column)
         it_behaves_like 'it updates downstream aliquots', [10, 11], insert_size_from: 6, insert_size_to: 7, i7: 2
 
         context 'when override is true' do
-          let(:override) { true }
+          let(:override_samples) { true }
 
           context 'when updating sample data' do
             let(:reupload) do
@@ -262,7 +263,7 @@ def cell(row, column)
         end
 
         context 'when override is false' do
-          let(:override) { false }
+          let(:override_samples) { false }
           let(:reupload) do
             SampleManifestExcel::Upload::Base.new(
               file: new_test_file,
@@ -511,7 +512,7 @@ def cell(row, column)
 
           after { FileUtils.rm_f(new_test_file_name) }
 
-          it 'will update the aliquots downstream if aliquots data has changed and override is set to true' do
+          it 'will update the aliquots downstream if aliquots data has changed and override is set to overall all' do
             cell(10, 7).value = '100'
             cell(11, 8).value = '1000'
             download.save(new_test_file_name)
@@ -520,7 +521,7 @@ def cell(row, column)
                 file: new_test_file,
                 column_list: column_list,
                 start_row: 9,
-                override: true
+                override: overrides
               )
             processor = described_class.new(reupload)
             processor.update_samples_and_aliquots(nil)
@@ -529,7 +530,7 @@ def cell(row, column)
             expect(processor).to be_downstream_aliquots_updated
           end
 
-          it 'will update the aliquots downstream if tag indexes were swapped and override is set to true' do
+          it 'will update the aliquots downstream if tag indexes were swapped and override is set to overall all' do
             tag_group1 = cell(10, 2).value
             tag_index1 = cell(10, 3).value
             tag_group2 = cell(11, 2).value
@@ -544,7 +545,7 @@ def cell(row, column)
                 file: new_test_file,
                 column_list: column_list,
                 start_row: 9,
-                override: true
+                override: overrides
               )
             processor = described_class.new(reupload)
             processor.update_samples_and_aliquots(nil)
@@ -558,7 +559,7 @@ def cell(row, column)
                 file: new_test_file,
                 column_list: column_list,
                 start_row: 9,
-                override: true
+                override: overrides
               )
             processor = described_class.new(reupload)
             processor.update_samples_and_aliquots(nil)
@@ -693,7 +694,7 @@ def cell(row, column)
                 file: new_test_file,
                 column_list: column_list,
                 start_row: 9,
-                override: true
+                override: overrides
               )
             processor = described_class.new(reupload)
             processor.update_samples_and_aliquots(nil)