diff --git a/api/controllers/UploadController.js b/api/controllers/UploadController.js
index fed2ba2e..9d85c38d 100644
--- a/api/controllers/UploadController.js
+++ b/api/controllers/UploadController.js
@@ -34,6 +34,7 @@ exports.headerValues = [
                     patientIdTypesSpecResult,
                     capturePanelResult,
                     readLengthsResult,
+                    squareSizesResult,
                 ] = results;
                 let currentApplications = applicationsResult.filter(
                     (application) => !constants.deprecatedApplications.includes(application.toLowerCase())
@@ -47,6 +48,7 @@ exports.headerValues = [
                     patientIdTypes: patientIdTypesResult,
                     patientIdTypesSpecified: patientIdTypesSpecResult,
                     readLengths: readLengthsResult,
+                    squareSizes: squareSizesResult,
                 };
                 return apiResponse.successResponseWithData(res, 'Operation success', responseObject);
             })
diff --git a/api/models/SubmissionModel.js b/api/models/SubmissionModel.js
index ac6a420b..51cf8e08 100644
--- a/api/models/SubmissionModel.js
+++ b/api/models/SubmissionModel.js
@@ -19,6 +19,7 @@ var FormSchema = new mongoose.Schema({
     serviceId: { type: String, required: true },
     species: { type: String, required: true },
     sequencingReadLength: { type: String, required: false },
+    squareSize: { type: String, required: false },
 });
 
 var SubmissionSchema = new mongoose.Schema(
diff --git a/api/util/constants.js b/api/util/constants.js
index f1ee561e..b3765ddb 100644
--- a/api/util/constants.js
+++ b/api/util/constants.js
@@ -1,6 +1,6 @@
 export const constants = {
     containers: ['Plates', 'Micronic Barcoded Tubes', 'Blocks/Slides/Tubes'],
-    headerPicklists: ['Recipe', 'Exemplar+Sample+Types', 'Species', 'PatientIDTypes', 'PatientIdTypesSpecified', 'Bait+Selection+Choices', 'Illumina+Sequencing+Run+Types'],
+    headerPicklists: ['Recipe', 'Exemplar+Sample+Types', 'Species', 'PatientIDTypes', 'PatientIdTypesSpecified', 'Bait+Selection+Choices', 'Illumina+Sequencing+Run+Types', 'Square+Size'],
     containersByMaterial: {
         Tissue: ['Blocks/Slides/Tubes', 'Micronic Barcoded Tubes'],
         Cells: ['Plates', 'Blocks/Slides/Tubes'],
diff --git a/api/util/helpers.js b/api/util/helpers.js
index 013574b1..5de78894 100644
--- a/api/util/helpers.js
+++ b/api/util/helpers.js
@@ -663,6 +663,7 @@ export function submit(submission, user, transactionId) {
         let capturePanel = submission.formValues.capturePanel;
         let sampleType = submission.formValues.material;
         let seqReadLength = submission.formValues.sequencingReadLength || '';
+        let squareSize = submission.formValues.squareSize || '';
         let samples = submission.gridValues;
         let submittedSamples = [];
         // prep banked sample record
@@ -678,6 +679,7 @@ export function submit(submission, user, transactionId) {
             bankedSample.user = process.env.API_USER;
             bankedSample.concentrationUnits = 'ng/uL';
             bankedSample.sequencingReadLength = seqReadLength;
+            bankedSample.squareSize = squareSize;
 
             if (recipe.includes('COVID')) {
                 bankedSample.userId = `${bankedSample.userId}-${serviceId}`;
diff --git a/frontend/src/components/Upload/UploadForm.js b/frontend/src/components/Upload/UploadForm.js
index fb98cd0a..bef5feca 100644
--- a/frontend/src/components/Upload/UploadForm.js
+++ b/frontend/src/components/Upload/UploadForm.js
@@ -26,6 +26,7 @@ class UploadForm extends React.Component {
                 patientIdTypeSpecified: true,
                 sharedWith: true,
                 sequencingReadLength: true,
+                squareSize: true,
             },
         };
     }
@@ -49,6 +50,9 @@ class UploadForm extends React.Component {
                 this.state.values.patientIdType === 'Both MSK-Patients and Non-MSK Patients')
         );
     };
+    showSquareSpaceDropdown = () => {
+        return this.state.values.application === '10X_Genomics_Visium';
+    };
     showReadLengthDropdown = () => {
         // dont show anything until they select application
         if (this.state.values.application.length === 0) return false;
@@ -152,6 +156,19 @@ class UploadForm extends React.Component {
                     formValid[value] = isValidOption && values[value].length > 0;
                     break;
 
+                case 'squareSize':
+                    if (!this.showSquareSpaceDropdown()) {
+                        formValid[value] = true;
+                        values[value] = '';
+                        break;
+                    }
+                    isValidOption = this.props.form.squareSizes.some(function(el) {
+                        return el === values[value];
+                    });
+
+                    formValid[value] = isValidOption && values[value].length > 0;
+                    break;
+
                 case 'sequencingReadLength':
                     if (!this.showReadLengthDropdown()) {
                         formValid[value] = true;
@@ -270,7 +287,8 @@ class UploadForm extends React.Component {
             this.state.formValid.patientIdTypeSpecified &&
             this.state.formValid.capturePanel &&
             this.state.formValid.sharedWith &&
-            this.state.formValid.sequencingReadLength
+            this.state.formValid.sequencingReadLength &&
+            this.state.formValid.squareSize
         );
     }
 
@@ -283,6 +301,7 @@ class UploadForm extends React.Component {
             handleMaterialChange,
             handleSpeciesChange,
             handleReadLengthChange,
+            handleSquareSizeChange,
             gridIsLoading,
             nothingToChange,
             gridNumberOfSamples,
@@ -329,6 +348,24 @@ class UploadForm extends React.Component {
                                     label: form.selected.application,
                                 }}
                             />
+                            {this.showSquareSpaceDropdown() && (
+                                <Dropdown
+                                    id='squareSize'
+                                    error={!formValid.squareSize}
+                                    onSelect={handleSquareSizeChange}
+                                    onChange={this.handleDropdownChange}
+                                    items={form.squareSizes.map((option) => ({
+                                        value: option,
+                                        label: option,
+                                    }))}
+                                    loading={form.formIsLoading}
+                                    dynamic
+                                    value={{
+                                        value: form.selected.squareSize,
+                                        label: form.selected.squareSize,
+                                    }}
+                                />
+                            )}
                             {this.showReadLengthDropdown() && (<Dropdown
                                 id='sequencingReadLength'
                                 error={!formValid.sequencingReadLength}
@@ -538,6 +575,7 @@ UploadForm.defaultProps = {
 
         allSpecies: [{ id: 'test', value: 'test' }],
         readLengths: [{ id: 'test', value: 'test' }],
+        squareSizes: [{ id: 'test', value: 'test' }],
         selected: {
             application: '',
             material: '',
@@ -548,6 +586,7 @@ UploadForm.defaultProps = {
             patientIdType: '',
             groupingChecked: false,
             sequencingReadLength: '',
+            squareSize: '',
             // altServiceId: false,
         },
 
diff --git a/frontend/src/containers/Upload/UploadFormContainer.js b/frontend/src/containers/Upload/UploadFormContainer.js
index 14eec05b..a829b34d 100644
--- a/frontend/src/containers/Upload/UploadFormContainer.js
+++ b/frontend/src/containers/Upload/UploadFormContainer.js
@@ -35,10 +35,15 @@ export class UploadFormContainer extends Component {
         }
     };
 
+    handleSquareSizeChange = (selectedSquareSize) => {
+        const { clearSquareSize } = this.props;
+        if (!selectedSquareSize) clearSquareSize();
+    };
+
     handleReadLengthChange = (selectedReadLength) => {
         const { clearReadLengths } = this.props;
         if (!selectedReadLength) clearReadLengths();
-    }
+    };
 
     handleSpeciesChange = (selectedSpecies) => {
         const { clearSpecies } = this.props;
@@ -78,6 +83,7 @@ export class UploadFormContainer extends Component {
                             handleApplicationChange={this.handleApplicationChange}
                             handleSpeciesChange={this.handleSpeciesChange}
                             handleReadLengthChange={this.handleReadLengthChange}
+                            handleSquareSizeChange={this.handleSquareSizeChange}
                             handleInputChange={this.handleInputChange}
                             handleClear={this.handleClear}
                         />
diff --git a/frontend/src/redux/actions/upload/formActions.js b/frontend/src/redux/actions/upload/formActions.js
index ded2dfe4..8b56d6ff 100644
--- a/frontend/src/redux/actions/upload/formActions.js
+++ b/frontend/src/redux/actions/upload/formActions.js
@@ -82,7 +82,7 @@ export const SELECT = 'SELECT';
 
 export function select(id, value, checkForMismatch = true) {
     return (dispatch) => {
-        if (id === 'species' || id === 'container' || id === 'patientIdType' || id === 'patientIdTypeSpecified' || id === 'sequencingReadLength') {
+        if (id === 'species' || id === 'container' || id === 'patientIdType' || id === 'patientIdTypeSpecified' || id === 'sequencingReadLength' || id === 'squareSize') {
             checkForMismatch && dispatch(checkForChange(id, value));
             return dispatch({
                 type: SELECT,
@@ -185,7 +185,12 @@ export const clearSpecies = () => {
 export const CLEAR_READ_LENGTHS = 'CLEAR_READ_LENGTHS';
 
 export const clearReadLengths = () => {
-    return [{ type: CLEAR_READ_LENGTHS }, { type: CLEARED }];;
+    return [{ type: CLEAR_READ_LENGTHS }, { type: CLEARED }];
+};
+
+export const CLEAR_SQUARE_SIZE = 'CLEAR_SQUARE_SIZE';
+export const clearSquareSize = () => {
+    return [{ type: CLEAR_SQUARE_SIZE }, { type: CLEARED }];
 };
 
 export const CLEAR_MATERIAL = 'CLEAR_MATERIAL';
diff --git a/frontend/src/redux/reducers/initialState.js b/frontend/src/redux/reducers/initialState.js
index 9207a82d..62f49b72 100644
--- a/frontend/src/redux/reducers/initialState.js
+++ b/frontend/src/redux/reducers/initialState.js
@@ -11,6 +11,7 @@ export const initialFormState = {
     patientIdTypes: [],
     patientIdTypesSpecified: [],
     readLengths: [],
+    squareSizes: [],
     allSpecies: [],
     filteredSpecies: [],
 
@@ -29,6 +30,7 @@ export const initialFormState = {
         isShared: false,
         sharedWith: '',
         sequencingReadLength: '',
+        squareSize: '',
     },
     // selected: {
     //   application: 'MouseWholeGenome',
diff --git a/frontend/src/redux/reducers/upload/formReducer.js b/frontend/src/redux/reducers/upload/formReducer.js
index 08fd4835..aa38fbaf 100644
--- a/frontend/src/redux/reducers/upload/formReducer.js
+++ b/frontend/src/redux/reducers/upload/formReducer.js
@@ -27,6 +27,7 @@ export default function formReducer(state = initialFormState, action) {
                 patientIdTypes: action.form_data.patientIdTypes,
                 patientIdTypesSpecified: action.form_data.patientIdTypesSpecified,
                 readLengths: action.form_data.readLengths,
+                squareSizes: action.form_data.squareSizes,
             };
 
         case ActionTypes.RECEIVE_INITIAL_STATE_FAIL:
@@ -228,6 +229,12 @@ export default function formReducer(state = initialFormState, action) {
                 selected: { ...state.selected, application: '' },
                 formIsLoading: true,
             };
+        case ActionTypes.CLEAR_SQUARE_SIZE:
+            return {
+                ...state,
+                selected: { ...state.selected, squareSize: '' },
+                formIsLoading: true,
+            };
         case ActionTypes.CLEAR_READ_LENGTHS:
             return {
                 ...state,
diff --git a/frontend/src/util/translations/en.json b/frontend/src/util/translations/en.json
index 262bb6ec..78ca9b85 100644
--- a/frontend/src/util/translations/en.json
+++ b/frontend/src/util/translations/en.json
@@ -23,6 +23,8 @@
         "groupIdType_label": "Patient or Group  ID Type*",
         "sequencingReadLength_label": "Sequencing Read Length*",
         "sequencingReadLength_helptext": "Select Read Length",
+        "squareSize_label": "Square Size*",
+        "squareSize_helptext": "Which size square would you like for each sample?",
 
         "isShared_label": "I want others to be able to edit or submit these samples on my behalf.",
         "sharedWith_label": "Give permission to:",